PrimePCR™ Probe Assay: LOS4, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LOS4, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LOS4, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0011488
Assay Design:   Exonic
Chromosome Location:   3:19688006-19688143question
Amplicon Length:   108
Splice Variants Targeted:   AT3G53110.1

Gene Information

Encodes a putative DEAD-Box RNA Helicase and has RNA-dependent ATPase activity. Mutant is Sensitive to chilling stress and heat stress. Germination of the mutant is inhibited by ABA. LOS4 may be involved in temperature sensing. Is enriched in the nuclear envelope and also located in the cytoplasm. LOS4 is involved in export of poly A RNA.

Gene Symbol:   LOS4
Gene Name:   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Aliases:   Not Available
RefSeq:   NM_115171
Ensembl:   AT3G53110
Entrez:   824477
Chromosome Mapping:   Not Available

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