PrimePCR™ Probe Assay: CHLI1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CHLI1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHLI1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0010274
Assay Design:   Exonic
Chromosome Location:   4:10202165-10202314question
Amplicon Length:   120
Splice Variants Targeted:   AT4G18480.1

Gene Information

Encodes the CHLI subunit of magnesium chelatase which is required for chlorophyll biosynthesis. All four cysteine residues of the protein form two disulfide bonds (Cys102-Cys193 and Cys354-Cys396) under oxidized conditions but are fully reduced by reduction. It was suggested that the redox state of CHLI is regulated in vivo by the change of the redox environment in the chloroplasts probably via the Trx system.

Gene Symbol:   CHLI1
Gene Name:   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Aliases:   Not Available
RefSeq:   NM_117962
Ensembl:   AT4G18480
Entrez:   827580
Chromosome Mapping:   Not Available

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