PrimePCR™ Probe Assay: MMZ1, Arabidopsis


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0009954
Assay Design:   Exonic
Chromosome Location:   1:8258571-8258678question
Amplicon Length:   78
Splice Variants Targeted:   AT1G23260.1

Gene Information

MMZ1/UEV1A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UEV1A can form diubiquitin and triubiquitin chains in combination with UBC13A/UBC35 in vitro. It can also functionally complement an mms2 mutation in budding yeast, both by increasing mms2 mutant viability in the presence of the DNA damaging agent MMS, and by reducing the rate of spontaneous DNA mutation. However, a combination of MMZ1/UEV1A and UBC13A do not do a good job of rescuing an mms2 ubc13 double mutant in yeast. MMZ1/UEV1A transcripts are found at low levels in most plant organs, but cannot be detected in the pollen. Transcript levels do not appear to be stress-inducible. The uev1a-1 mutant shows normal sensitivity to MMS in germination assays suggesting that UEV1A is not required for DNA damage tolerance during this developmental stage.

Gene Symbol:   MMZ1
Gene Name:   MMS ZWEI homologue 1
Aliases:   Not Available
RefSeq:   NM_102175
Ensembl:   AT1G23260
Entrez:   838935
UniGene:   At.15639
Chromosome Mapping:   Not Available

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