PrimePCR™ Probe Assay: COW1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COW1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COW1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0005564
Assay Design:   Exonic
Chromosome Location:   4:16518080-16518198question
Amplicon Length:   89
Splice Variants Targeted:   AT4G34580.1

Gene Information

Encodes COW1 (can of worms1), a phosphatidylinositol transfer protein essential for root hair tip growth. The N-terminus of the COW1 protein is 32% identical to an essential phosphatidylinositol transfer protein (PITP), the yeast Sec14 protein (sec14p) while the C-terminus is 34.5% identical to a late nodulin of Lotus japonicus, Nlj16. Expression of COW1 complements the growth defect associated with Sec14p dysfunction in yeast. GFP fused to the COW1 protein specifically accumulates at the site of root hair outgrowth.

Gene Symbol:   COW1
Gene Name:   Sec14p-like phosphatidylinositol transfer family protein
Aliases:   Not Available
RefSeq:   NM_119624
Ensembl:   AT4G34580
Entrez:   829610
Chromosome Mapping:   Not Available

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