PrimePCR™ Probe Assay: CYP704B1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CYP704B1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYP704B1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0003026
Assay Design:   Exonic
Chromosome Location:   1:26125219-26125336question
Amplicon Length:   88
Splice Variants Targeted:   AT1G69500.1

Gene Information

Encodes a cytochrome P450, designated CYP704B1. Expressed in the developing anthers. Essential for pollen exine development. Mutations in CYP704B1 result in impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface, termed zebra phenotype. Heterologous expression of CYP704B1 in yeast cells demonstrated that it catalyzes omega-hydroxylation of long-chain fatty acids, implicating these molecules in sporopollenin synthesis.

Gene Symbol:   CYP704B1
Gene Name:   Cytochrome P450, family 704, subfamily B, polypeptide 1
Aliases:   Not Available
RefSeq:   NM_105617
Ensembl:   AT1G69500
Entrez:   843283
Chromosome Mapping:   Not Available

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