PrimePCR™ SYBR® Green Assay: AOP3, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: AOP3, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AOP3, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0053279
Assay Design:   Exonic
Chromosome Location:   4:1345907-1346040question
Amplicon Length:   104
Splice Variants Targeted:   AT4G03050.1 AT4G03050.2

Gene Information

Transcriptional silent in leaf tissues of ecotype Col. The transcribed allele in ecotype Ler encodes a 2-oxoglutarate-dependent dioxygenase which is involved in glucosinolate biosynthesis. The natural variation in this locus explains the diversification of hydroxyalkyl glucosinolates among different ecotypes of Arabidopsis.

Gene Symbol:   AOP3
Gene Name:   2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Aliases:   Not Available
RefSeq:   NM_116539 NM_001036503
Ensembl:   AT4G03050
Entrez:   828104
Chromosome Mapping:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download