PrimePCR™ SYBR® Green Assay: PHS1, Arabidopsis

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0052895
Assay Design:   Exonic
Chromosome Location:   3:17459356-17459474question
Amplicon Length:   89
Splice Variants Targeted:   AT5G65410.1 AT3G47390.1 AT3G47390.2

Gene Information

Encodes a protein that is believed to function as a pyrimidine reductase involved in riboflavin and FAD biosynthesis. phs1 was identified as a photosensitive mutant that shows reduced growth, chloroplast developmental abnormalities, reduced chlorophyll levels, increased oxidative stress, reduced NADPH/NADP+ ratios, reduced photosystem I electron transport, and reduced photosynthetic protein levels under high light conditions. Many of these abnormal phenotypes likely arise from the reduction in the levels of FAD in the phs1 mutant.

Gene Symbol:   PHS1
Gene Name:   Cytidine/deoxycytidylate deaminase family protein
Aliases:   Not Available
RefSeq:   NM_001203097 NM_114607
Ensembl:   AT3G47390
Entrez:   823893
UniGene:   At.53819
Chromosome Mapping:   Not Available

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
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LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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