PrimePCR™ SYBR® Green Assay: ARC5, Arabidopsis

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0052624
Assay Design:   Exonic
Chromosome Location:   3:6850931-6851137question
Amplicon Length:   81
Splice Variants Targeted:   AT3G19720.1 AT3G19720.2 AT3G19720.3

Gene Information

Encodes a novel chloroplast division protein. Mutants of exhibit defects in chloroplast constriction, have enlarged, dumbbell-shaped chloroplasts. The ARC5 gene product shares similarity with the dynamin family of GTPases, which mediate endocytosis, mitochondrial division, and other organellar fission and fusion events in eukaryotes. Phylogenetic analysis showed that ARC5 is related to a group of dynamin-like proteins unique to plants. A GFP ARC5 fusion protein localizes to a ring at the chloroplast division site. Chloroplast import and protease protection assays indicate that the ARC5 ring is positioned on the outer surface of the chloroplast. Facilitates separation of the two daughter chloroplasts.

Gene Symbol:   ARC5
Gene Name:   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Aliases:   Not Available
RefSeq:   NM_180284 NM_112862 NM_001203006
Ensembl:   AT3G19720
Entrez:   821509
UniGene:   At.38361
Chromosome Mapping:   Not Available

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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