PrimePCR™ SYBR® Green Assay: SPY, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: SPY, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SPY, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0052442
Assay Design:   Exonic
Chromosome Location:   3:3632203-3632306question
Amplicon Length:   74
Splice Variants Targeted:   AT4G00760.1 AT4G00770.1 AT3G11540.1 AT3G11540.2

Gene Information

Encodes a N-acetyl glucosamine transferase that may glycosylate other molecules involved in GA signaling. Contains a tetratricopeptide repeat region, and a novel carboxy-terminal region. SPY acts as both a repressor of GA responses and as a positive regulation of cytokinin signalling. SPY may be involved in reducing ROS accumulation in response to stress.

Gene Symbol:   SPY
Gene Name:   Tetratricopeptide repeat (TPR)-like superfamily protein
Aliases:   Not Available
RefSeq:   NM_111987 NM_202557
Ensembl:   AT3G11540
Entrez:   820327
Chromosome Mapping:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download