PrimePCR™ SYBR® Green Assay: ROL5, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ROL5, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ROL5, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0052153
Assay Design:   Exonic
Chromosome Location:   2:18297947-18298153question
Amplicon Length:   177
Splice Variants Targeted:   AT4G29080.1 AT2G44270.1 AT2G44270.2

Gene Information

Encodes ROL5, a repressor of lrx1 mutants that develop aberrant root hairs. ROL5 is a homolog of yeast Nc.6 that affects TOR signaling. The target of rapamycin (TOR) pathway is a major regulator of cell growth in eukaryotes, and inhibition of this pathway by rapamycin reduces cell growth. ROL5 might function as a mitochondrial component of the TOR pathway that influences the plant's response to ROS (reactive oxygen species).

Gene Symbol:   ROL5
Gene Name:   Repressor of lrx1
Aliases:   Not Available
RefSeq:   NM_001202817 NM_129991
Ensembl:   AT2G44270
Entrez:   819035
Chromosome Mapping:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download