PrimePCR™ SYBR® Green Assay: TIM, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0051660
Assay Design:   Exonic
Chromosome Location:   2:9072960-9073078question
Amplicon Length:   89
Splice Variants Targeted:   AT5G11730.1 AT2G21170.1 AT2G21170.2

Gene Information

Encodes a plastidic triose phosphate isomerase. Mutants with reduced pdTPI levels have difficulty transitioning from heterotrophic to autotrophic growth. The related phenotypes, such as chlorosis in light-grown seedlings may result from an accumulation of dihydroxyacetone phosphate (DHAP) and methylglyoxal (MG) in these mutants. Both splice variants appear to be expressed, but the At2g21170.2 variant appears to have a much narrower expression range limited to roots.

Gene Symbol:   TIM
Gene Name:   Triosephosphate isomerase
Aliases:   Not Available
RefSeq:   NM_001084462 NM_127687
Ensembl:   AT2G21170
Entrez:   816652
UniGene:   At.24668,At.31909
Chromosome Mapping:   Not Available

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