PrimePCR™ SYBR® Green Assay: WIT2, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0051154
Assay Design:   Exonic
Chromosome Location:   1:25909582-25909694question
Amplicon Length:   83
Splice Variants Targeted:   AT4G14190.1 AT1G68910.1 AT1G68910.2 AT1G68910.3

Gene Information

Encodes one of the WPP domain-interacting proteins (WIT1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). WIT1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

Gene Symbol:   WIT2
Gene Name:   WPP domain-interacting protein 2
Aliases:   Not Available
RefSeq:   NM_105565 NM_001198424 NM_001084328
Ensembl:   AT1G68910
Entrez:   843224
UniGene:   At.35468
Chromosome Mapping:   Not Available

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