PrimePCR™ SYBR® Green Assay: 4CL3, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0051079
Assay Design:   Exonic
Chromosome Location:   1:24168806-24170317question
Amplicon Length:   81
Splice Variants Targeted:   AT1G65060.1 AT1G65060.2

Gene Information

encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. mRNA levels are not induced in response to wounding or to fungal infection by P. parasitica. mRNA is expressed in flowers, to a lesser degree in mature leaves and siliques and marginally in seedling roots and bolting stems of mature plants. The catalytic efficiency was in the following (descending) order: p-coumaric acid, caffeic acid, ferulic acid, cinnamic acid and 5-OH-ferulic acid. At4CL3 was unable to use sinapic acid as substrate.

Gene Symbol:   4CL3
Gene Name:   4-coumarate:CoA ligase 3
Aliases:   Not Available
RefSeq:   NM_105180 NM_179513
Ensembl:   AT1G65060
Entrez:   842814
UniGene:   At.11514
Chromosome Mapping:   Not Available

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