PrimePCR™ SYBR® Green Assay: VIM1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0050938
Assay Design:   Exonic
Chromosome Location:   1:21417575-21417690question
Amplicon Length:   86
Splice Variants Targeted:   AT2G30990.1 AT2G30990.2 AT2G30990.3 AT1G57820.1 AT1G57820.2

Gene Information

Encodes a 645-amino acid methylcytosine-binding protein with a PHD domain, two RING finger domains, and an SRA domain that is involved in centromere heterochromatinization. This protein functions as an E3 ubiquitin ligase in vitro. The protein has been shown to bind to methylated cytosines of CG, CNG and CNN motifs via its SRA domain but has a preference for the former. It plays a role in the establishment/maintenance of chromatin structure during cell division and is localized in the nucleus. Plants over-expressing VIM1/ORTH2 show an inhibition in root growth and a delay in flowering. Both over-expression of GFP:ORTH2 and loss of ORTH2/VIM1 lead to decreased levels of DNA methylation. GFP:ORTH2 over-expressers also have increased levels of FWA transcripts.

Gene Symbol:   VIM1
Gene Name:   Zinc finger (C3HC4-type RING finger) family protein
Aliases:   Not Available
RefSeq:   NM_104576 NM_202316
Ensembl:   AT1G57820
Entrez:   842157
UniGene:   At.28484
Chromosome Mapping:   Not Available

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