PrimePCR™ SYBR® Green Assay: ATU2AF35A, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0050522
Assay Design:   Exonic
Chromosome Location:   1:9615643-9615783question
Amplicon Length:   111
Splice Variants Targeted:   AT5G42820.1 AT5G42820.2 AT1G27650.1 AT1G27650.2

Gene Information

U2 auxiliary factor small subunit. The atU2AF35a protein and its homolog, atU2AF35b, contain most of the conserved domains of hsU2AF35, including the psiRRM, one RS domain, two zinc fingers, and the two regions for interacting with U2AF large subunit. Both proteins lack the stretch of glycines present in human U2AF35. The sequences are overall 83% identical, and each Arabidopsis homolog shows approximately 70% similarity to hsU2AF35. U2AF(35) homologs were also identified from maize, rice and other plants with large-scale EST projects. Both genes are expressed in all major tissues, with atU2AF(35)a expressed at a higher level than atU2AF(35)b in most tissues. The expression patterns were different in roots: atU2AF(35)b expressed strongly in whole young roots and root tips and atU2AF(35)a limited to root vascular regions.

Gene Symbol:   ATU2AF35A
Gene Name:   U2 snRNP auxiliary factor small subunit, putative
Aliases:   Not Available
RefSeq:   NM_102530 NM_001084140
Ensembl:   AT1G27650
Entrez:   839657
UniGene:   At.20200,At.63995,At.67851
Chromosome Mapping:   Not Available

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