PrimePCR™ SYBR® Green Assay: PFT1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: PFT1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PFT1, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0050497
Assay Design:   Exonic
Chromosome Location:   1:8971748-8972199question
Amplicon Length:   123
Splice Variants Targeted:   AT3G06530.1 AT3G06530.2 AT3G06530.3 AT1G25540.1 AT1G25540.2

Gene Information

Encodes a nuclear protein that acts in a phyB pathway (but downstream of phyB) and induces flowering in response to suboptimal light conditions. Mutants are hypo-responsive to far-red and hyper-responsive to red light and flower late under long day conditions. Also shown to be a Mediator subunit regulating jasmonate-dependent defense.

Gene Symbol:   PFT1
Gene Name:   Phytochrome and flowering time regulatory protein (PFT1)
Aliases:   Not Available
RefSeq:   NM_001084127 NM_102365
Ensembl:   AT1G25540
Entrez:   839141
Chromosome Mapping:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download