PrimePCR™ SYBR® Green Assay: RPT5B, Arabidopsis

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0044172
Assay Design:   Exonic
Chromosome Location:   1:2939186-2939301question
Amplicon Length:   86
Splice Variants Targeted:   AT1G09100.1

Gene Information

Encodes RPT5b (Regulatory Particle 5b), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.

Gene Symbol:   RPT5B
Gene Name:   26S proteasome AAA-ATPase subunit RPT5B
Aliases:   Not Available
RefSeq:   NM_100781
Ensembl:   AT1G09100
Entrez:   837431
UniGene:   At.10379,At.42241
Chromosome Mapping:   Not Available

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