PrimePCR™ SYBR® Green Assay: WAG2, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0040574
Assay Design:   Exonic
Chromosome Location:   3:4798177-4798285question
Amplicon Length:   79
Splice Variants Targeted:   AT3G14370.1

Gene Information

The WAG2 and its homolog, WAG1 each encodes protein-serine/threonine kinase that are nearly 70% identical to PsPK3 protein. All three together with CsPK3 belong to PsPK3-type kinases. At the N-terminus, all four possess a serine/threonine-rich domain. They are closely related to Arabidopsis kinases PINOID. wag1/wag2 double mutants exhibit a pronounced wavy root phenotype when grown vertically on agar plates (while wild-type plants develop wavy roots only on plates inclined to angles less than 90 degrees), indicating an overlapping role for WAG1 and WAG2 as suppressors of root waving. Simultaneous disruption of PID(AT2G34650) and its 3 closest homologs (PID2/AT2G26700, WAG1/AT1G53700, and WAG2/AT3G14370) abolishes the formation of cotyledons.

Gene Symbol:   WAG2
Gene Name:   Protein kinase superfamily protein
Aliases:   Not Available
RefSeq:   NM_112295
Ensembl:   AT3G14370
Entrez:   820658
UniGene:   At.39249
Chromosome Mapping:   Not Available

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