PrimePCR™ SYBR® Green Assay: GAPA, Arabidopsis

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0039936
Assay Design:   Exonic
Chromosome Location:   3:9796662-9796794question
Amplicon Length:   103
Splice Variants Targeted:   AT3G26650.1

Gene Information

Encodes one of the two subunits forming the photosynthetic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and as such a constituent of the supramolecular complex with phosphoribulokinase (PRK) thought to be linked by a small peptide encoded by CP12-2. GapA-1 is coordinately expressed by light with PRK and CP12-2. The enzyme activity, tested in leaf protein extracts dropped significantly after external sucrose treatment for the photosynthetic GAPDH (NADPH-dependent) but not for the cytosolic GAPDH (NADH-dependent).

Gene Symbol:   GAPA
Gene Name:   Glyceraldehyde 3-phosphate dehydrogenase A subunit
Aliases:   Not Available
RefSeq:   NM_113576
Ensembl:   AT3G26650
Entrez:   822277
UniGene:   At.3405,At.20459,At.23357
Chromosome Mapping:   Not Available

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
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LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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