PrimePCR™ SYBR® Green Assay: GAPA, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: GAPA, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GAPA, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0039936
Assay Design:   Exonic
Chromosome Location:   3:9796662-9796794question
Amplicon Length:   103
Splice Variants Targeted:   AT3G26650.1

Gene Information

Encodes one of the two subunits forming the photosynthetic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and as such a constituent of the supramolecular complex with phosphoribulokinase (PRK) thought to be linked by a small peptide encoded by CP12-2. GapA-1 is coordinately expressed by light with PRK and CP12-2. The enzyme activity, tested in leaf protein extracts dropped significantly after external sucrose treatment for the photosynthetic GAPDH (NADPH-dependent) but not for the cytosolic GAPDH (NADH-dependent).

Gene Symbol:   GAPA
Gene Name:   Glyceraldehyde 3-phosphate dehydrogenase A subunit
Aliases:   Not Available
RefSeq:   NM_113576
Ensembl:   AT3G26650
Entrez:   822277
Chromosome Mapping:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download