PrimePCR™ SYBR® Green Assay: CER10, Arabidopsis

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0039726
Assay Design:   Exonic
Chromosome Location:   3:20521393-20521492question
Amplicon Length:   70
Splice Variants Targeted:   AT3G55360.1

Gene Information

Enoyl-CoA reductase is involved in all very long chain fatty acids (VLCFA) elongation reactions that are required for cuticular wax, storage lipid and sphingolipid metabolism. The protein is located in the ER, but in contrast to its yeast homolog TSC13 is not particularly enriched in the nuclear envelope-vacuole junction. Mutants in this gene show abnormal organ morphology and stem glossiness. Cells in all tissues are only about 1/3 of the size of wild type cells. The morphological changes are most likely to result from the reduction in the VLCFA content of sphingolipids. Mutants also show abnormalities in the endocytic membrane organization and transport.

Gene Symbol:   CER10
Gene Name:   3-oxo-5-alpha-steroid 4-dehydrogenase family protein
Aliases:   Not Available
RefSeq:   NM_115394
Ensembl:   AT3G55360
Entrez:   824702
UniGene:   At.23806
Chromosome Mapping:   Not Available

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