PrimePCR™ SYBR® Green Assay: ANNAT1, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0038112
Assay Design:   Exonic
Chromosome Location:   1:13226742-13226844question
Amplicon Length:   73
Splice Variants Targeted:   AT1G35720.1

Gene Information

Encodes a member of the annexin gene family, a diverse, multigene family of calcium-dependent, membrane-binding proteins. The protein was determined to have peroxidase activity. This activity is thought to be dependent on the presence of post-translational modifications (most likely phosphorylation). The protein was shown to be present as a mixture of monomer and homodimer. The homodimerization seems to be dependent on the presence of Ca2+ or H2O2. The dimerization was prevented by the addition of DTT, β-mercaptoethanol and TCEP. Annat1 mRNA is expressed in flowers, roots,leaves and stems and is most abundant in stems. mRNA levels are increased in response to oxidative stress. Developmental expression patterns suggest a role in Golgi-mediated polysaccharide secretion.

Gene Symbol:   ANNAT1
Gene Name:   Annexin 1
Aliases:   Not Available
RefSeq:   NM_103274
Ensembl:   AT1G35720
Entrez:   840476
UniGene:   At.25241,At.50678
Chromosome Mapping:   Not Available

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