PrimePCR™ SYBR® Green Assay: MEA, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0037187
Assay Design:   Exonic
Chromosome Location:   1:546526-546744question
Amplicon Length:   100
Splice Variants Targeted:   AT1G02580.1

Gene Information

Encodes a putative transcription factor MEDEA (MEA) that negatively regulates seed development in the absence of fertilization. Mutations in this locus result in embryo lethality. MEA is a Polycomb group gene that is imprinted in the endosperm. The maternal allele is expressed and the paternal allele is silent. MEA is controlled by DEMETER (DME), a DNA glycosylase required to activate MEA expression, and METHYLTRANSFERASE I (MET1), which maintains CG methylation at the MEA locus. MEA is involved in the negative regulation of its own imprinted gene expression; the effect is not only allele-specific but also dynamically regulated during seed development. In the ovule, the MEA transcripts are accumulated at their highest level before fertilization and gradually decrease after fertilization

Gene Symbol:   MEA
Gene Name:   SET domain-containing protein
Aliases:   Not Available
RefSeq:   NM_100139
Ensembl:   AT1G02580
Entrez:   839422
UniGene:   At.10786
Chromosome Mapping:   Not Available

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