PrimePCR™ SYBR® Green Assay: TPS02, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0033791
Assay Design:   Exonic
Chromosome Location:   4:9405721-9405831question
Amplicon Length:   81
Splice Variants Targeted:   AT4G16730.1

Gene Information

In the Col ecotype, no functional protein is encoded at this locus due to the presence of a two-base (AT) insertion 184 nucleotides downstream of the start codon leading to a frame shift and premature translational termination. However, in the Ws ecotype, a functional terpene synthase that localizes to the chloroplast is encoded at this locus. It can catalyze the synthesis of (E)-beta-ocimene and (E,E)-alpha farnesene in vitro, but, it has more activity as an (E)-beta-ocimene synthase activity in vivo. This may reflect the greater availability of the GPP precursor of (E)-beta-ocimene than of the FPP precursor of (E,E)-alpha-farnesene in the chloroplasts where the Ws TPS02 is present.

Gene Symbol:   TPS02
Gene Name:   Terpene synthase 02
Aliases:   Not Available
RefSeq:   NM_117774
Ensembl:   AT4G16730
Entrez:   827376
UniGene:   At.54364
Chromosome Mapping:   Not Available

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