PrimePCR™ SYBR® Green Assay: TPS02, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $133.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: TPS02, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TPS02, Arabidopsis
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0033791
Assay Design:   Exonic
Chromosome Location:   4:9405721-9405831question
Amplicon Length:   81
Splice Variants Targeted:   AT4G16730.1

Gene Information

In the Col ecotype, no functional protein is encoded at this locus due to the presence of a two-base (AT) insertion 184 nucleotides downstream of the start codon leading to a frame shift and premature translational termination. However, in the Ws ecotype, a functional terpene synthase that localizes to the chloroplast is encoded at this locus. It can catalyze the synthesis of (E)-beta-ocimene and (E,E)-alpha farnesene in vitro, but, it has more activity as an (E)-beta-ocimene synthase activity in vivo. This may reflect the greater availability of the GPP precursor of (E)-beta-ocimene than of the FPP precursor of (E,E)-alpha-farnesene in the chloroplasts where the Ws TPS02 is present.

Gene Symbol:   TPS02
Gene Name:   Terpene synthase 02
Aliases:   Not Available
RefSeq:   NM_117774
Ensembl:   AT4G16730
Entrez:   827376
Chromosome Mapping:   Not Available

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download