This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: TPS02, Arabidopsis
PrimePCR™ Template for SYBR® Green Assay: TPS02, Arabidopsis
In the Col ecotype, no functional protein is encoded at this locus due to the presence of a two-base (AT) insertion 184 nucleotides downstream of the start codon leading to a frame shift and premature translational termination. However, in the Ws ecotype, a functional terpene synthase that localizes to the chloroplast is encoded at this locus. It can catalyze the synthesis of (E)-beta-ocimene and (E,E)-alpha farnesene in vitro, but, it has more activity as an (E)-beta-ocimene synthase activity in vivo. This may reflect the greater availability of the GPP precursor of (E)-beta-ocimene than of the FPP precursor of (E,E)-alpha-farnesene in the chloroplasts where the Ws TPS02 is present.