PrimePCR™ SYBR® Green Assay: CLPB2, Arabidopsis

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qAthCED0032975
Assay Design:   Exonic
Chromosome Location:   4:8411928-8412048question
Amplicon Length:   91
Splice Variants Targeted:   AT4G14670.1

Gene Information

This locus was originally annotated as encoding ClpB2 (also referred to as Hsp92.7), which belongs to the Casein lytic proteinase/heat shock protein 100 (Clp/Hsp100) family. However, according to Lee et al. (2007, Plant Journal, 49:115-127), there is no evidence for expression of an appropriate-sized mRNA from this locus. Re-annotation of the genome indicates that this locus potentially encodes a 68.8-kDa protein, containing only the N-terminal two thirds of the originally predicted open reading frame. This locus contains a 626-bp deletion in WS ecotype compared with the Col ecotype, which eliminates residues 1-86 of the predicted protein.

Gene Symbol:   CLPB2
Gene Name:   Casein lytic proteinase B2
Aliases:   Not Available
RefSeq:   NM_117549
Ensembl:   AT4G14670
Entrez:   827117
UniGene:   At.54318
Chromosome Mapping:   Not Available

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