This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qRnoCED0011844; exonic
mast cell protease that acts as a beta-chymase; has a strong preference for bulky/aromatic amino acid residues in both the P1 and P2 positions [RGD, Feb 2006]