This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qRnoCED0005015; exonic
converts 5,10 methylenetetrahydrofolate to 5-methyltetrahydrofolate; mutations in human may be associated with hyperhomocysteinaemia, and a risk factor for spina bifida in women [RGD, Feb 2006]