This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qRnoCED0001646; exonic
binds the carbohydrate responsive element and regulates transcription of 1-type pyruvate kinase gene (1-PK); DNA binding activity is inhibited by AMP-activated protein kinase (AMPK) phosphorylation of Ser596 [RGD, Feb 2006]