This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qMmuCID0021544; Intron-spanning
This gene encodes a member of the Ste20 family of serine/threonine protein kinases and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1 and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq Jul 2008]