This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qMmuCID0015005; Intron-spanning
The protein encoded by this gene is part of the tripartite-motif containing family (TRIM) which is typified by the RING B-box type 1 B-box type 2 and coiled-coil region domains. In mouse this protein is proposed to oligomerize through its coiled coil domain and has been reported to be expressed in neural crest-derived tissues as well as in tissues whose development is regulated by mesenchymal-epithelial interactions. In humans mutations in this gene are associated with mulibrey (muscle-liver-brain-eye) nanism an autosomal recessive disorder characterized by prenatal onset growth failure cardiomyopathy and dysmorphic features. [provided by RefSeq Jan 2013]