PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Dio1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qMmuCED0049506; exonic

List Price:    $130.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dMmuEG5069446
Chromosome Location:   4:107292422-107293858question
Amplicon Length:   81

Gene Information

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 33'5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). This protein contains a selenocysteine (Sec) residue which is necessary for maximal enzyme activity. The selenocysteine is encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Impaired expression of this gene results in hypothyroidism which causes severe defects in the development of many different tissues. [provided by RefSeq Jul 2008]

Gene Symbol:   Dio1
Gene Name:   Deiodinase, iodothyronine, type I
Aliases:   5DI, D1, ITDI1, TXDI1
RefSeq:   NC_000070.6 NT_039264.7
Ensembl:   ENSMUSG00000034785
Entrez:   13370
UniGene:   Mm.148342
Chromosome Mapping:   4 C7|4 48.7 cM

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