This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qMmuCED0048780; exonic
This gene encodes a selenoprotein which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure the sec insertion sequence (SECIS) that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. The exact function of this protein is not known however it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR) an endoplasmic reticulum(ER)-resident protein which is involved in the quality control of protein folding. The association with UGTR retains this selenoprotein in the ER where it may play a role in protein folding. [provided by RefSeq Jul 2008]