This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: HEX; Same primer pair and probe as used in qPCR assay qMmuCIP0034291; Intron-spanning
This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains which bind microtubules and regulate microtubule polymerization a C-terminal serine/threonine protein kinase domain which shows substantial homology to Ca2+/calmodulin-dependent protein kinase and a serine/proline-rich domain in between the doublecortin and the protein kinase domains which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes including neuronal migration retrograde transport neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found but the biological validity of some variants has not been determined. These variants encode different isoforms which are differentially expressed and have different kinase activities. [provided by RefSeq Sep 2010]