PrimePCR™ ddPCR™ Expression Probe Assay: Tyms, Mouse

PrimePCR

ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; Same primer pair and probe as used in qPCR assay qMmuCEP0054588; exonic

 
Fluorophore:   HEX
List Price:    $201.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dMmuCPE5096295
Chromosome Location:   5:30059068-30059200question
Amplicon Length:   103

Gene Information

This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 510-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294 3444407). The stop codon in these transcripts is UAA compared to the UAG found in the genome and longer transcripts as the polyA site is located within the stop codon (PMID: 3444407 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq Mar 2010]

Gene Symbol:   Tyms
Gene Name:   Thymidylate synthase
Aliases:   Ts
RefSeq:   NC_000071.6 NT_165760.3
Ensembl:   ENSMUSG00000025747
Entrez:   22171
UniGene:   Mm.268395
Chromosome Mapping:   5 B1|5 18.2 cM

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