PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.R68T, Human

PrimePCR

ddPCR probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   100nt amplicon; exonic; in silico

 
Fluorophore:   HEX
List Price:    $335.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Mutation Detection in silico design
Unique Assay ID:   dHsaIS2504859
Chromosome Location:   1:115256447-115256569question
Amplicon Length:   100
Restriction Enzymes:   HaeIII

Gene Information

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein which has intrinsic GTPase activity is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer follicular thyroid cancer autoimmune lymphoproliferative syndrome Noonan syndrome and juvenile myelomonocytic leukemia. [provided by RefSeq Jun 2011]

Gene Symbol:   NRAS
Gene Name:   neuroblastoma RAS viral (v-ras) oncogene homolog
Aliases:   ALPS4, N-ras, NRAS1, NS6
RefSeq:   NC_000001.10 NG_007572.1 NT_032977.9
Ensembl:   ENSG00000213281
Entrez:   4893
UniGene:   Hs.486502
Chromosome Mapping:   1p13.2

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