PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.D281Y, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   64nt amplicon; exonic; in silico

 
Fluorophore:   HEX
List Price:    $325.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Mutation Detection in silico design
Unique Assay ID:   dHsaIS2503427
Chromosome Location:   17:7577036-7577158question
Amplicon Length:   64
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes tumor protein p53 which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest apoptosis senescence DNA repair or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation DNA-binding and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms which can regulate p53 transcriptional activity. [provided by RefSeq Jul 2008]

Gene Symbol:   TP53
Gene Name:   tumor protein p53
Aliases:   FLJ92943, LFS1, P53, TRP53
RefSeq:   NC_000017.10 NG_017013.1 NT_010718.16
Ensembl:   ENSG00000141510
Entrez:   7157
UniGene:   Hs.437460
Chromosome Mapping:   17p13.1

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