PrimePCR™ ddPCR™ Mutation Assay:VHL WT for p.R161*, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   65nt amplicon; crosses exon-intron junction; in silico

 
Fluorophore:   HEX
List Price:    $325.00
Your Price:   Log In
 

Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Mutation Detection in silico design
Unique Assay ID:   dHsaIS2503095
Chromosome Location:   3:10191427-10191549question
Amplicon Length:   65
Restriction Enzymes:   HaeIII

Gene Information

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B elongin C and cullin-2 and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF) which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq Jul 2008]

Gene Symbol:   VHL
Gene Name:   von Hippel-Lindau tumor suppressor
Aliases:   HRCA1, RCA1, VHL1
RefSeq:   NC_000003.11 NG_008212.3 NT_022517.18
Ensembl:   ENSG00000134086
Entrez:   7428
UniGene:   Hs.517792
Chromosome Mapping:   3p25.3

Number Description Download
10033487 ddPCR™ Mutation Detection Assays Product Insert, Ver C Click to download
10033173 ddPCR™ Copy Number Variation Assays Product Insert, Ver C Click to download
10041702 PrimePCR™ ddPCR™ Gene Expression Assays (EvaGreen) Product Insert, Rev A Click to download
10047489 ddPCR™ Mutation Detection Assays Product Insert, Ver B Click to download
10048730 PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.