This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: 65nt amplicon; exonic; in silico
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats and an intracellular domain consisting of multiple different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia notch interaction with its cell-bound ligands (delta serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands and may play multiple roles during development. [provided by RefSeq Jul 2008]