PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HLA-DRB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0037245; Intron-spanning

List Price:    $134.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5189850
Chromosome Location:   6:32546835-32548601question
Amplicon Length:   120

Gene Information

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB) both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3 DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3 DRB4 and DRB5. There are 4 related pseudogenes: DRB2 DRB6 DRB7 DRB8 and DRB9. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DRB1
Gene Name:   major histocompatibility complex, class II, DR beta 1
Aliases:   DRB1, DRw10, FLJ75017, FLJ76359, HLA-DR1B, HLA-DRB, HLA-DRB1*, SS1
RefSeq:   NC_000006.11 NG_002392.2 NG_002432.1 NG_002433.1 NT_007592.15 NT_113891.2 NT_167249.1
Ensembl:   ENSG00000196126
Entrez:   3123
UniGene:   Hs.696211
Chromosome Mapping:   6p21.3

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