PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NANS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0020983; Intron-spanning

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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5189732
Chromosome Location:   9:100840571-100843171question
Amplicon Length:   103

Gene Information

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) respectively; however it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq Jul 2008]

Gene Symbol:   NANS
Gene Name:   N-acetylneuraminic acid synthase
Aliases:   SAS
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000095380
Entrez:   54187
UniGene:   Hs.522310
Chromosome Mapping:   9p24.1-p23

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