PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PYGL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017715; Intron-spanning

List Price:    $134.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5189671
Chromosome Location:   14:51390793-51398477question
Amplicon Length:   83

Gene Information

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-14-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver brain and muscle respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI also known as Hers disease mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia mild ketosis growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq Feb 2011]

Gene Symbol:   PYGL
Gene Name:   phosphorylase, glycogen, liver
Aliases:   GSD6
RefSeq:   NC_000014.8 NT_026437.12 NG_012796.1
Ensembl:   ENSG00000100504
Entrez:   5836
UniGene:   Hs.282417
Chromosome Mapping:   14q21-q22

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