PrimePCR™ ddPCR™ Expression EvaGreen® Assay: AKT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0011338; Intron-spanning

List Price:    $134.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5189575
Chromosome Location:   14:105237099-105238788question
Amplicon Length:   143

Gene Information

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Jul 2011]

Gene Symbol:   AKT1
Gene Name:   v-akt murine thymoma viral oncogene homolog 1
Aliases:   AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
RefSeq:   NC_000014.8 NT_026437.12 NG_012188.1
Ensembl:   ENSG00000142208
Entrez:   207
UniGene:   Hs.525622
Chromosome Mapping:   14q32.32,14q32.32

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