This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0045475; exonic
This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. FXYD4 originally named CHIF for channel-inducing factor has been shown to modulate the properties of the NaK-ATPase as has FXYD2 also known as the gamma subunit of the NaK-ATPase and FXYD7. Transmembrane topology has been established for FXYD4 and two family members (FXYD1 and FXYD2) with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. Alternatively spliced transcript variants encoding the same protein have been found.[provided by RefSeq May 2010]