This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0038249; exonic
The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA) angiotensinogen (AGT) and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage exfoliation and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]