PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HLA-B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037470; exonic

List Price:    $134.00
Your Price:   Log In
 

Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5188582
Chromosome Location:   6:31321920-31322010question
Amplicon Length:   61

Gene Information

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide exon 2 and 3 encode the alpha1 and alpha2 domains which both bind the peptide exon 4 encodes the alpha3 domain exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-B
Gene Name:   major histocompatibility complex, class I, B
Aliases:   AS, Bw-45, Bw-48, Bw-56, HLA-B-5601, HLA-B-6701, HLA-B27, HLA-B73, HLAB, MGC111087, SPDA1
RefSeq:   NC_000006.11 NG_002397.2 NG_023187.1 NT_007592.15 NT_113891.2 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000234745
Entrez:   3106
UniGene:   Hs.77961
Chromosome Mapping:   6p21.3

Number Description Download
10033487 ddPCR™ Mutation Detection Assays Product Insert, Ver C Click to download
10033173 ddPCR™ Copy Number Variation Assays Product Insert, Ver C Click to download
10041702 PrimePCR™ ddPCR™ Gene Expression Assays (EvaGreen) Product Insert, Rev A Click to download
10047489 ddPCR™ Mutation Detection Assays Product Insert, Ver B Click to download
10048730 PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A Click to download