This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0037275; exonic
This gene is part of a region that is repeated three times on chromosome X once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the middle copy. Although its function is unknown the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII this gene is transcribed abundantly in a wide variety of cell types. [provided by RefSeq Jul 2008]