This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0020487; Exonic
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate a methyl group shuttle required for the de novo synthesis of purines thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5 multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq Jul 2008]