PrimePCR™ ddPCR™ Expression EvaGreen® Assay: TNNT3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCED0005032; Exonic

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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5188055
Chromosome Location:   11:1955776-1955868question
Amplicon Length:   63

Gene Information

The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which ultimately produces a muscle contraction. The troponin complex has protein subunits C I and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal- slow skeletal- and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq Oct 2009]

Gene Symbol:   TNNT3
Gene Name:   troponin T type 3 (skeletal, fast)
Aliases:   DKFZp779M2348, TNTF
RefSeq:   NC_000011.9 NT_009237.18 NG_013085.1
Ensembl:   ENSG00000130595
Entrez:   7140
UniGene:   Hs.73454
Chromosome Mapping:   11p15.5

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