This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0038566; Intron-spanning
Ceramidases (EC 126.96.36.199) such as ASAH2 catalyze hydrolysis of the N-acyl linkage of ceramide a second messenger in a variety of cellular events to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al. 2001 [PubMed 11328816]).[supplied by OMIM Mar 2008]