This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0037882; Intron-spanning
This gene encodes a member of the GIT protein family which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes focal adhesions and the cell periphery and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq Sep 2008]