This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0036694; Intron-spanning
This gene encodes a pyrin-like protein containing a pyrin domain a nucleotide-binding site (NBS) domain and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation the immune response and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS) Muckle-Wells syndrome (MWS) chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq Oct 2008]